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And by the 1990s, drostanolone propionate also became popular among bodybuilders who were looking to get the perfect physique before a bodybuilding competition (3)In the same year, there emerged a brand of anti-doping supplements called Dianabol that was made by a Czech scientist. These drugs were first developed by Peter C. D'Agostino and were introduced in the late 1960s by Dr. Dosty, who later published more scientific research on the treatment of anabolic steroid abuse (4). By 1974, when this anti-testosterone regimen was first prescribed for female athletes in the United States, Dianabol had grown to encompass a wide range of supplements, including testosterone creams, and had been approved by the United States Food and Drug Administration to contain testosterone (5). In 1976, it was discovered that the use of Dianabol was associated with serious adverse effects, including liver, kidney, and thyroid disorders, heart and blood vessel injuries, depression, and sexual problems (6).As an anti-testosterone regimen, Dianabol was more toxic than other amphetamine-like stimulants (7), but unlike amphetamine, it did not require much concentration compared to the amount of urine required to deliver Dianabol (8). Dosty eventually began using this regimen for his athletes (9). By 1976, the US Anti-Doping Agency banned him for three years for violating the drug-testing rules because of this abuse, but not before he was able to obtain his approval of an accelerated restart of Dianabol use in the United States (10). It was around this time that the anti-doping methods that Dr. Robert Gallo and Dr. D'Agostino had developed were introduced into North America through the use of anti-deoxyribonucleic acid (DNA) testing at the World Anti-Doping Agency (WADA).DNA testing has only recently caught up with biological substances in use today. However, while DNA tests have the potential to detect banned substances, the lack of a standardized set of requirements, and the fact that testing by the US Anti-Doping Agency (USADA) is not standardized (11), has led to uncertainty in the testing of human growth hormone (HGH), as well as the use of the newer synthetic steroids.USADA's Guidelines for the Testing of Human Growth Hormone on Steroid UsersWADA's guidelines for the use, administration and testing of human growth hormone have evolved significantly over the years, and currently the guidelines follow:Each positive result should be reported immediately and a urine specimen should be collected from the subject within the prescribed time frame (typically sixSimilar articles: